PCR: Need for Speed?

Enhancing speed has been one key objective for cycler manufacturers since then. Fortunately, the components needed for the heat transfer in a PCR instrument became better and better in quality and performance, i.e. Peltier elements and thermoblock materials. Taking advantage of major technological advances over the years, Eppendorf succeeded in developing a PCR cycler which is probably the fastest model on the market: Mastercycler^® X50s. It features a silver block for fast heat transfer and Peltier elements of excellent quality enabling PCR runs with heating rates of 10 °C/s. Its innovative 2D-Gradient technology allows testing of 96 different combinations of denaturation and annealing temperatures in one run in order to optimize yield and specificity of a PCR. This does not speed up the run itself, but the process to determine the optimal conditions for the DNA and primer pair takes less time. PCR kit manufacturers, too, dedicate their expertise to enhancing the speed of PCR. Their aim is to find more reliable, stable, and fast enzymes for PCR preparation. Thanks to new technologies and kits, the runtime of a PCR can nowadays be reduced from 1 hour down to 15 minutes while keeping the same quality of results. Make the most of your working day and spend the time you win with your PCR results and new findings! Speed up your PCR with Mastercycler X50s + Fast PCR enzymes, e.g. from Solis BioDyne.

Related links

Mastercycler X50 website
Online article “Multiple targets, one run: multiplex your PCR!”
Online article “Higher faster further”

Related documents

Product Brochure Mastercycler Magazine | BioNews 60 (Jan 2024)